RNA labelling techniques: (a) FRET. (b) Fluorophore & quencher. (c) FIT-PNA. (d) Sticky-flare. (e) Molecular beacon probe. (f) Wavelength-shifting molecular beacon. (g) Dual molecular beacon FRET probes. (h) MS2 system. (i) Spinach aptamer (D: Doner of FRET pair; A: Accpetor of FRET pair; Q: Quencher; TO: Thiazole Orange; DFHBI: 3,5-difluoro-4-hydroxybenzylidene imidazolinone.). Image (c) IOP Publishing, All Rights Reserved.
This week, our Image of the Week comes from a recent Journal of Physics D: Applied Physics Topical Review studying the state-of-the-art techniques which are transforming the understanding of mRNA in living cells.
Organic dyes and fluorescent proteins are used to label mRNA for tracking. Our image is a schematic of a range of different processes used to attach these labels and ensure that they are active only when bound to the target RNA molecule under investigation.
This work is licensed under a Creative Commons Attribution 3.0 Unported License
Image from Hyungseok C Moon et al 2016 J. Phys. D: Appl. Phys. 49 233001, Copyright IOP Publishing, All Rights Reserved.
Categories: Journal of Physics D: Applied Physics
Image of the Week: Flexible, polarization-controllable diffusion metasurface with optical transparency 
Quantifying plasma chemistry products at atmospheric pressure 
Image of the Week: Direct writing using 3D printed, melt electrospun fibres 
Image of the week: Coalescence of Pickering emulsions. 
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